Dynamics and Interactions of Bio-Nano-structures

Molecular interactions of bio-nanostructures and the accompanying dynamics form the basis of all biological processes and are thus of great im-portance from a pharmaceutical and medical perspective. In order to understand these processes as well as their potential malfunction, it is necessary to exam-ine and characterize their nanoscopic roots in detail.

For this purpose, the TIMed CENTER Core Facility ‘Dynamics and Interactions of Bio-Nanostructures’ uses high-speed atomic force microscopy (HS-AFM). a technique that allows the label-free examination of protein dynamics, molecular interactions and conformational changes under physiological condi-tions, in real-time and at sub-molecular resolution.

The combination of fluorescence and atomic force microscopy (FM‐AFM) further allows the manipulation of cells as well as the defined transport of bio‐molecules such as active substances. In addition to imaging techniques, biophysical single-molecule forces (force spectroscopy) are used to determine binding forces or to physically characterise surfaces. Ensemble methods are also used here to quantify intermolecular interactions and chemical rates. Based on the information obtained, a comprehensive model of a molecular process is created, which is used in drug development.

Functions and Services

• Analysis of molecular interactions as well as physical surface properties by means of force spectroscopy, quartz crystal microbalance (QCM), surface plasmon resonance imaging (SPRi)
• Chemical rate constants and affinities, stoichiometry, multivalence, interaction forces and energies
• Direct and label‐free visualization of biomolecules, interactions as well as conformational changes by means of high‐speed atomic force microscopy; cellular interaction studies in high‐resolution both in terms of time and space (characterization of active ingredients) (FM‐AFM)
• Combination of results and model building, mathematical modelling and simulation

Contact

E nano@timed-center.at